Analysis Note

Positive Control293 cells, HeLa cells, Human breast carcinoma tissue

Application

ELISA (see coments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (10 g/ml)

General description

Anti-FASN, mouse monoclonal, clone 3F2-1F3, recognizes FASN protein in 293 and HeLa cells and in human breast carcinoma tissue. It is validated for ELISA, WB, ICC, and IHC (paraffin sections).

Purified mouse monoclonal antibody. Recognizes the FASN protein.

Recognizes the FASN protein in 293 and HeLa cells and human breast carcinoma tissue.

Immunogen

Full-length, recombinant human FASN (aa 1-440) expressed as GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Kristiansen, G., et al. 2010. Br. J. Cancer102, 1736.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)